A simple fluorescent probe based on a pyrene derivative for rapid detection of protamine and monitoring of trypsin activity.
نویسندگان
چکیده
We report the synthesis of a simple pyrene derivative and its application in protamine detection and monitoring of trypsin activity. This assay can be conducted in aqueous solution and features rapid response, visual detection, high sensitivity and selectivity. The limit of detection of protamine was 0.5 μg mL(-1). The IC50 value of a soybean trypsin inhibitor was estimated to be 0.51 U mL(-1).
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ورودعنوان ژورنال:
- Organic & biomolecular chemistry
دوره 13 32 شماره
صفحات -
تاریخ انتشار 2015